FastQCFastQC Report
Tue 23 Jul 2013
A22-03-R2_val_2.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-03-R2_val_2.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 17038723
Filtered Sequences 0
Sequence length 20-101
%GC 38

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 121845 0.7151064079156636 No Hit
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 103441 0.6070936184595523 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 86073 0.5051610968732809 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 70426 0.4133290974916371 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 48255 0.2832078436864077 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 44989 0.2640397405368935 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 37761 0.2216187210743434 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 33433 0.1962177564598004 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 30354 0.17814715339876117 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 28946 0.1698836233208322 No Hit
CTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGAA 28841 0.16926737995564572 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 28299 0.16608639039439752 No Hit
CTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATTTA 27248 0.15991808775810254 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 25115 0.1473995439681718 No Hit
AATTAATAAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGC 23523 0.13805612075505894 No Hit
CCTCGATGTTGAATTAAAATAAAAATTAGATGTAGATGTCTAATATTTAG 23057 0.1353211740105171 No Hit
CAAGTTTTTAATTAAAAAACAATTGATTATGCTACCTTTGCACAGTCATT 23050 0.13528009111950468 No Hit
TATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAA 21726 0.12750955573372488 No Hit
GGCAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATA 21253 0.12473352609817061 No Hit
CTTAAACCTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTG 20036 0.117590972046438 No Hit
GCTACCTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGG 19901 0.1167986591483411 No Hit
CCTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGA 19858 0.11654629281783618 No Hit
TTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCTT 19725 0.11576571788859999 No Hit
GTCTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATT 19193 0.11264341817165524 No Hit
CTCCCAACTAAATATAATTCATATTATTAATGATACAAAAATTTTTAATA 18747 0.11002585111572036 No Hit
CCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTAAA 18160 0.10658075725510649 No Hit
GTTGCTTTTAGCCCTTGAGATCCGTGAATTTGTTAAATCCGTCAGTTGTT 18013 0.10571801654384547 No Hit
GTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATG 17887 0.1049785245056217 No Hit
AAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTG 17509 0.10276004839095043 No Hit
TTATAATTAAGAAAGAATTAATTACCTTAGGGATAACAGCGTAATATTTT 17352 0.1018386178353859 No Hit
TAAAAACATAGCTTTTAGATTATAATTTAAGGTTATTTCTGCCCCATGAG 17231 0.10112847071931388 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGGCC 1540980 3.5588174 8.238988 1
GGCCG 1431680 3.3858502 5.4986815 9
GCGGC 1405375 3.32364 7.6834226 70-74
GGTCG 1673725 2.5048037 6.389794 85-89
CACCG 1709605 2.4591293 13.12261 1
GGCCA 1659290 2.4441113 5.145349 75-79
CGGCG 1029385 2.434443 8.909226 1
CGCCG 1040110 2.4020824 8.128046 1
CTGGC 1629780 2.381801 10.534665 1
CGATC 2598655 2.3653858 12.036004 4
CCGGC 937205 2.1644282 7.558115 1
CCTCG 1500230 2.1410222 6.9759183 1
GGCTT 2247960 2.0788963 6.7617297 3
CCGAT 2259965 2.0570984 10.605834 3
GGGGC 815350 1.9745991 13.105465 95-97
CTGCG 1340325 1.958784 5.0947533 70-74
TGGCT 2060060 1.9051279 6.571275 45-49
CGACG 1283020 1.8898706 5.792522 1
GACCT 2068595 1.8829067 8.475262 95-97
ACCGA 2019770 1.853009 8.555841 2
GCCGA 1253380 1.8462114 5.5004687 1
AGGTC 1943625 1.8116693 5.413034 95-97
CGCGG 763115 1.804728 5.858549 1
CCGCG 778935 1.7989117 5.5263753 1
GCAAA 3051780 1.7857426 5.335418 2
CAGCG 1128030 1.6615725 6.9843245 1
GTCGC 1084985 1.585624 7.216504 95-97
ATATT 6938235 1.5751244 5.1770897 4
CGCAA 1686995 1.5477093 5.2687106 1
CTTTC 2757075 1.5386307 9.939292 1
GATCT 2610670 1.5037414 7.4953003 5
CGAAC 1597520 1.4656216 6.2620845 90-94
TCCTT 2548340 1.4221427 7.7188306 2
CCTTT 2535250 1.4148376 7.749255 3
CTAAA 3843710 1.3898594 6.0313015 8
CAAAT 3600435 1.3018928 5.84746 9
AGACC 1401140 1.2854556 7.1858554 95-97
ACACC 1392520 1.247567 5.504711 8
CAGAC 1355005 1.2431297 6.7746177 95-97
CTCGT 1323095 1.194874 5.098711 1
CTCGG 809365 1.1828259 5.7573805 1
CTCAT 2096160 1.1790506 6.79535 1
TTTCG 1955090 1.1172893 8.301423 2
CGTAC 1220535 1.1109732 12.3053 5
TTCGT 1941900 1.1097516 8.2058 3
CTCCC 731140 1.018945 6.031819 1
GTCCT 1019275 0.92049724 11.142479 1
GGGTC 600530 0.8987197 5.2622423 2
TCGTA 1557020 0.8968408 7.886001 7
GTACA 1531680 0.88922447 8.417034 6
ACAAA 2313985 0.8433407 5.463969 8
TACAA 2204235 0.7970364 5.8930507 7