FastQCFastQC Report
Tue 23 Jul 2013
A22-24-R1_val_1.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-24-R1_val_1.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 17868629
Filtered Sequences 0
Sequence length 20-101
%GC 38

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 92065 0.5152325900325089 No Hit
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 80698 0.4516183082652844 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 65663 0.3674764303405706 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 57508 0.3218377862118017 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 44652 0.2498904644558908 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 43992 0.24619684028360542 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 36009 0.2015207769997351 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 31378 0.17560384739086585 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 30034 0.16808228544003012 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 27839 0.15579818686705063 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 25689 0.1437659263058179 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 22093 0.12364127096712345 No Hit
TTATAATTAAGAAAGAATTAATTACCTTAGGGATAACAGCGTAATATTTT 21472 0.12016590640501854 No Hit
CTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGAA 20553 0.11502281456512416 No Hit
TTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCTT 20482 0.11462547014659043 No Hit
CCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTAAA 18901 0.10577756133388858 No Hit
CCTCGATGTTGAATTAAAATAAAAATTAGATGTAGATGTCTAATATTTAG 18741 0.10488213729212242 No Hit
AATTAATAAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGC 18383 0.10287862599867063 No Hit
GTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATG 18323 0.10254284198300831 No Hit
CAAGTTTTTAATTAAAAAACAATTGATTATGCTACCTTTGCACAGTCATT 18275 0.10227421477047847 No Hit
TATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAA 18146 0.10155227913680451 No Hit
AATTAATTACCTTAGGGATAACAGCGTAATATTTTTAGAAAGATCATATT 18093 0.10125566992296947 No Hit
CTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATTTA 18080 0.10118291671957598 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGGCC 1655070 3.5611677 8.273124 1
GGCCG 1594670 3.4547062 6.1987696 9
GCGGC 1496260 3.2415104 6.5052857 70-74
CGGCG 1216570 2.6355875 8.981237 1
CGCCG 1203230 2.5889564 7.918634 1
GGTCG 1865740 2.5715625 6.527176 95-97
CACCG 1883245 2.5614655 13.624136 1
GCCGG 1124535 2.436202 5.60637 1
CTGGC 1774850 2.4296484 10.66466 1
CCGGC 1129085 2.4294207 8.540261 1
CCTCG 1782570 2.4236174 6.6448636 1
CGATC 2779305 2.4050472 12.459572 4
TGGCC 1622125 2.2205784 5.198074 8
GACCT 2402935 2.0793586 8.328574 95-97
CGACG 1463350 2.0039835 5.8259873 1
CCGCG 927300 1.9952455 5.845528 1
CGCGG 916220 1.9849068 5.9315844 1
CCGAT 2281430 1.9742151 10.89616 3
AGGTC 2201115 1.9177604 5.5561857 95-97
GGGGC 866810 1.8907256 9.716423 95-97
ACCGA 2102040 1.8196696 8.938548 2
CAGCG 1315680 1.801757 7.0937147 1
GGCTT 2045240 1.781278 7.131742 3
GCCAG 1273265 1.7436718 5.2486897 1
GTCGC 1206190 1.6511917 7.3987045 95-97
TGGCT 1817670 1.5830786 5.753543 45-49
CTTTC 2796620 1.5286148 8.701222 1
CGAAC 1758935 1.5226543 5.5970683 90-94
GATCT 2755635 1.5171018 7.807658 5
TCCTT 2722630 1.4881723 7.0857954 2
CCTTT 2566105 1.4026167 7.0827045 3
AGACC 1532470 1.3266107 6.4173894 95-97
CAGAC 1483300 1.2840458 5.861175 95-97
CAAAT 3596490 1.2521077 5.216538 9
GCGTA 1415365 1.2331618 5.840808 1
CTCGG 895215 1.2254882 5.129092 1
ACACC 1424650 1.2248855 5.8058643 8
CTAAA 3336550 1.1616102 5.151899 8
CGTAC 1321880 1.1438773 10.660875 5
TTTCG 2064045 1.1359208 7.32338 2
TTCGT 2045460 1.1256927 7.33505 6
CTCCC 803945 1.0856245 5.070389 1
CCGGG 488155 1.0575432 5.3077455 1
CTCAT 1907930 1.0432566 5.1498647 1
GTCCT 1080890 0.93498504 9.908045 1
TCGTA 1653850 0.9105193 7.145583 7
GTACA 1594305 0.87806886 7.323728 6
TACAA 2192935 0.763464 5.2508254 7