FastQCFastQC Report
Tue 23 Jul 2013
A22-28-R1_val_1.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-28-R1_val_1.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13905764
Filtered Sequences 0
Sequence length 20-101
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 66821 0.4805273554189472 No Hit
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 55878 0.4018333692417044 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 46705 0.33586791779293823 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 42451 0.3052762868692436 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 29824 0.21447221454355186 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 28340 0.2038003809067952 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 28337 0.2037788071191198 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 22142 0.15922893556945164 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 20276 0.1458100396353627 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 20044 0.14414166672179968 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 18153 0.13054298922374924 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 16987 0.12215797708058328 No Hit
CTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGAA 14986 0.10776826070110208 No Hit
AATTAATAAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGC 14895 0.1071138558082821 No Hit
CAAGTTTTTAATTAAAAAACAATTGATTATGCTACCTTTGCACAGTCATT 14464 0.10401442164558523 No Hit
CTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATTTA 14179 0.10196491181642375 No Hit
TATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAA 14047 0.10101566515870684 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGGCC 1279400 3.2466478 7.4154534 1
GGCCG 1232345 3.1582327 5.313756 9
GCGGC 1195075 3.062718 6.314579 70-74
CGGCG 977490 2.5050948 8.369431 1
CGCCG 970955 2.4639275 7.569942 1
CACCG 1426955 2.3530715 10.821159 1
GGTCG 1366115 2.2904727 5.3213024 95-97
CGATC 2105945 2.2719455 10.790951 4
GCCGG 863530 2.2130399 5.3336973 1
CCTCG 1343355 2.2083228 6.245189 1
CCGGC 865180 2.1955094 7.462159 1
CTGGC 1318375 2.1887379 8.89103 1
CGACG 1186020 1.9751492 5.5604806 1
GGCTT 1791705 1.9460198 6.0130386 3
CCGAT 1768560 1.9079661 9.265651 3
CGCGG 725480 1.8592478 5.576798 1
CCGCG 731175 1.8554538 5.4921303 1
GACCT 1711415 1.8463167 7.386073 95-97
GCCGA 1087785 1.8115528 5.432373 1
TGGCT 1637730 1.7787833 5.3412404 45-49
GCAAA 2468030 1.7528046 5.059435 2
ACCGA 1580845 1.7107763 7.2679105 2
GGGGC 659040 1.7057158 9.278116 95-97
CAGCG 1010535 1.6829036 6.2442427 1
ATATT 5432830 1.6301405 5.089228 4
GTCGC 928635 1.5417 5.971051 95-97
CTTTC 2099180 1.4624816 8.329896 1
GATCT 2064125 1.4568433 6.870156 5
TCCTT 2069910 1.4420894 6.5048747 2
CGAAC 1294985 1.4014212 5.0885544 90-94
CCTTT 1960200 1.3656553 6.5071726 3
CTAAA 2947320 1.35598 5.6979938 8
CAAAT 2711185 1.2473408 5.003518 9
GCGTA 1128885 1.2299391 5.084173 1
AGACC 1126770 1.2193803 5.813542 95-97
CTCGG 725710 1.2048082 5.3034353 1
CAGAC 1069755 1.1576793 5.376628 95-97
TTTCG 1585175 1.1153238 6.9346385 2
TTCGT 1563890 1.1003478 6.8202767 3
CGTAC 1015375 1.0954115 9.783063 5
CTCAT 1561155 1.091038 5.556082 1
CTCCC 621460 1.0115838 5.1760406 1
GTCCT 851225 0.9154659 8.751115 1
TCGTA 1275635 0.9003332 6.496738 7
GTACA 1210780 0.8572257 6.964643 6
TACAA 1691835 0.77836627 5.113195 7
Produced by FastQC (version 0.10.1)