FastQCFastQC Report
Tue 23 Jul 2013
A22-38-R2_val_2.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-38-R2_val_2.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 19235179
Filtered Sequences 0
Sequence length 20-101
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 142028 0.7383762844109742 No Hit
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 126149 0.6558244142152251 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 89114 0.46328656468442536 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 64599 0.3358377897081176 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 49296 0.2562804328465048 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 48329 0.25125318563450855 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 40759 0.21189821004525095 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 35930 0.18679316683249997 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 29986 0.15589145284273154 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 29313 0.152392655145034 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 27715 0.14408496016595426 No Hit
CCTCGATGTTGAATTAAAATAAAAATTAGATGTAGATGTCTAATATTTAG 24021 0.12488056388765606 No Hit
TATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAA 23389 0.12159491731270086 No Hit
CCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTAAA 22899 0.11904750145553625 No Hit
AATTAATTACCTTAGGGATAACAGCGTAATATTTTTAGAAAGATCATATT 21938 0.1140514470907705 No Hit
TTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCTT 21886 0.11378110908143875 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 21493 0.11173797758783528 No Hit
AATTAATAAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGC 20794 0.10810401088547188 No Hit
TTATAATTAAGAAAGAATTAATTACCTTAGGGATAACAGCGTAATATTTT 20772 0.10798963711229305 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGGCC 1789300 2.9226892 6.3152275 1
GCGGC 1694185 2.823181 5.397974 1
GGTCG 2143515 2.435492 6.4505806 90-94
CGGCG 1461075 2.4347277 8.534476 1
CGATC 3241860 2.4305496 12.302326 4
CACCG 2189885 2.40796 12.629421 1
CGCCG 1460915 2.3862965 7.7930474 1
CTGGC 1998100 2.2253537 9.104851 45-49
CCTCG 1979975 2.1615393 6.5389085 1
GCCGG 1286220 2.1433501 5.1954427 1
CCGGC 1278010 2.087535 6.991285 1
CCGAT 2756550 2.066694 10.776421 3
GGCTT 2542110 1.9304495 5.8534307 3
CGACG 1715695 1.9246265 5.0508723 1
ACCGA 2471005 1.8659863 8.645701 2
CGCGG 1102455 1.8371252 5.499631 1
GACCT 2437795 1.8277109 7.840917 95-97
CCGCG 1116715 1.8240716 5.4529114 1
AGGTC 2382165 1.8220512 5.659388 95-97
TGGCT 2363170 1.7945645 6.471436 45-49
GCCGA 1564030 1.7544922 5.409355 1
GATCT 3204740 1.6382699 8.222689 5
CAGCG 1433840 1.6084481 6.373732 1
GTCGC 1416035 1.5770875 7.253781 95-97
CGAAC 2001205 1.5112156 6.3260865 90-94
TCCTT 3000510 1.4927418 9.041921 2
CTTTC 2980720 1.4828964 9.099937 4
GGGGC 867820 1.4753191 7.8939667 95-97
CTAAA 4088220 1.4170326 6.418431 8
CCTTT 2718515 1.3524506 8.924765 3
CAAAT 3840920 1.3313152 5.777865 9
AGACC 1650420 1.2463193 6.6045656 95-97
ATCTA 3556235 1.223803 5.0356207 6
CAGAC 1607610 1.2139912 6.31695 95-97
CTCGG 1079020 1.2017422 5.2132645 1
CGTAC 1582660 1.1865823 12.571984 8
TTCGT 2313270 1.1740708 9.285786 6
TTTCG 2297810 1.1662245 9.258261 5
GTCCT 1308415 0.9739378 12.339434 1
TCGTA 1901480 0.9720404 9.288748 7
GTACA 1804740 0.9292484 8.632555 9
ACAAA 2572650 0.89815414 5.4259725 8
TACAA 2394930 0.83011544 5.904102 7